Optimization of Cultural Conditions for Antioxidant Exopolysaccharides from Xerocomus badius Grown in Shrimp Byproduct

نویسندگان

  • Xiujun Gao
  • Peisheng Yan
  • Xin Liu
  • Jianbing Wang
  • Jiajia Yu
چکیده

To optimize the production conditions for exopolysaccharides with higher antioxidant activities from Xerocomus badius cultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of 4.588 ± 0.346 g/L and a total antioxidant activity of 2.956 ± 0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

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عنوان ژورنال:

دوره 2016  شماره 

صفحات  -

تاریخ انتشار 2016